edu.ucar.opensky::articles:11534 UCAR/NCAR - Library OpenSky Support PO Box 3000 Boulder CO 80307-3000 opensky@ucar.edu http://opensky.ucar.edu/ homepage pointOfContact 2025-12-26T14:07:54.201953 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) https://n2t.org/ark:/85065/d7c53md7 Analysis of stress-induced transcripts and biogenic volatile organic compound emissions in tropical hymenaea courbaril and coniferous pinus ponderosa 2010-12-01T00:00:00Z publication https://n2t.org/ark:/85065/d7c53md7 https://doi.org/10.1007/s11626-010-9339-6 Thornton, B. author Guenther, Alex B. author Harley, P. author Greenberg, James author Basu, C. author UCAR/NCAR - Library publisher Changes in the composition of biogenic volatile organic compound (BVOC) emissions and stress-responsive gene transcripts were investigated in the tropical tree species Hymenaea courbaril and the coniferous species Pinus ponderosa. Heat stress and insect wounding have been associated with an increase in the release of stored and de novo synthesized BVOCs. These BVOCs are released into the atmosphere, where they can interact with nitrous oxides to create ozone and alter cloud properties via the formation of secondary organic aerosols (SOAs). To determine the source of the BVOCs being released (stored vs. de novo), we subjected both species (Ponderosa and Hymenaea) to heat stress and methyl jasmonate (MeJa) treatment. The temperature in the growth chamber containing the trees was increased from 24°C to 40°C (Ponderosa) or 27°C to 42°C (Hymenaea), and BVOCs were collected using leaf cuvette enclosures. Leaves were collected at 0 and 24 h for transcript analysis. Heat- and MeJa-responsive gene transcripts were detected using SeeGene's GeneFishing™ technology. These differentially expressed genes were then classified using BLAST analysis, and expression of 25 selected gene transcripts were examined by qPCR. These stress-inducible genes have potentials to be transferred to plants for trait enhancements. UCAR/NCAR - Library OpenSky Support PO Box 3000 Boulder CO 80307-3000 opensky@ucar.edu http://opensky.ucar.edu/ homepage pointOfContact PDF Text theme Resource Type 2016-01-01T00:00:00Z publication Copyright. All rights reserved. University Corporation for Atmospheric Research (UCAR). The user is granted the right to use this resource for non-commercial, non-profit research, or educational purposes only, as are more fully described in the UCAR Terms of Use. None eng geoscientificInformation