Identification

Title

Analysis of stress-induced transcripts and biogenic volatile organic compound emissions in tropical hymenaea courbaril and coniferous pinus ponderosa

Abstract

Changes in the composition of biogenic volatile organic compound (BVOC) emissions and stress-responsive gene transcripts were investigated in the tropical tree species Hymenaea courbaril and the coniferous species Pinus ponderosa. Heat stress and insect wounding have been associated with an increase in the release of stored and de novo synthesized BVOCs. These BVOCs are released into the atmosphere, where they can interact with nitrous oxides to create ozone and alter cloud properties via the formation of secondary organic aerosols (SOAs). To determine the source of the BVOCs being released (stored vs. de novo), we subjected both species (Ponderosa and Hymenaea) to heat stress and methyl jasmonate (MeJa) treatment. The temperature in the growth chamber containing the trees was increased from 24°C to 40°C (Ponderosa) or 27°C to 42°C (Hymenaea), and BVOCs were collected using leaf cuvette enclosures. Leaves were collected at 0 and 24 h for transcript analysis. Heat- and MeJa-responsive gene transcripts were detected using SeeGene's GeneFishing™ technology. These differentially expressed genes were then classified using BLAST analysis, and expression of 25 selected gene transcripts were examined by qPCR. These stress-inducible genes have potentials to be transferred to plants for trait enhancements.

Resource type

document

Resource locator

Unique resource identifier

code

http://n2t.net/ark:/85065/d7c53md7

codeSpace

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

geoscientificInformation

Keywords

Keyword set

keyword value

Text

originating controlled vocabulary

title

Resource Type

reference date

date type

publication

effective date

2016-01-01T00:00:00Z

Geographic location

West bounding longitude

East bounding longitude

North bounding latitude

South bounding latitude

Temporal reference

Temporal extent

Begin position

End position

Dataset reference date

date type

publication

effective date

2010-12-01T00:00:00Z

Frequency of update

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Conformity

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Use constraints

Copyright. All rights reserved. University Corporation for Atmospheric Research (UCAR). The user is granted the right to use this resource for non-commercial, non-profit research, or educational purposes only, as are more fully described in the UCAR Terms of Use.

Limitations on public access

None

Responsible organisations

Responsible party

contact position

OpenSky Support

organisation name

UCAR/NCAR - Library

full postal address

PO Box 3000

Boulder

80307-3000

email address

opensky@ucar.edu

web address

http://opensky.ucar.edu/

name: homepage

responsible party role

pointOfContact

Metadata on metadata

Metadata point of contact